Gallery
Outstanding Image of the Month:
Courtesy of Rosedelma Díaz, graduate student of the UPR-RP Department of Biology.
Expression of AChR ε/GFP subunit fusion polypeptide upon electroporation in the mice muscle. The pRK5-ε/GFP DNA was electroporated in the tibialis anterior muscle of 3-month-all FVB mice. Fourteen days later a longitudinal 50 µm section of the tibialis muscle was analyzed using confocal fluorescence images at the neuromuscular junction (NMJ). The muscle was stained with α-bungatotoxin Alexa Fluor 647 (α-BTX) to mark synapse (magenta), DAPI to mark nuclei (blue), Phalloidin Alexa Fluor 555 to mark actin filaments. (A) ε/GFP polypeptides expressed in the muscle (green). (B) AChR at the NMJ. (C) Overlay of ε/GFP subunit and α-BTX shows that ε/GFP subunits were colocalized with the AChR at the NMJ (gray), demonstrating that they were assembled into the receptor complex. Scale bar equal to 10 µm.